Becker & Hickl GmbH
Becker & Hickl have introduced a proprietary time-correlated single-photon counting principle that made TCSPC more than 100 times faster than the existing devices. Moreover, bh introduced a multi-dimensional TCSPC process that records the photons not only versus the time in the signal period, but also versus other parameters, such as experiment time, wavelength, or spatial coordinates. The bh devices are designed to record multi-dimensional photon distributions, time-resolved images, sequences of photon distributions, or multi-dimensional time-tag data. The bh TCSPC products are complemented by bh picosecond diode lasers, detector modules, multi-spectral detector assemblies, and experiment control modules. Based on these components Becker & Hickl supply their own confocal fluorescence lifetime laser scanning microscope and FLIM upgrade kits for laser scanning microscopes of various manufacturers. Moreover, bh is supplier of TCSPC modules for commercial time-domain optical tomography instruments. bh market activities include currently 5 workshops around the world and distribution of more than 1200 pages of TCSPC literature.
- +49 (30) 212 80 02-0
- +49 (30) 212 80 02-13
- info@becker-hickl.com
- Nunsdorfer Ring 7-9
Berlin, 12277
Germany
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Standard Lifetime Measurement System
Filter-Based Systems
*Excitation by bh ps Diode Lasers (BDS-SM or BDL-SMN Series)*Optional: fs Fiber Laser*Wavelength Selection by Filters*Detection by bh HPM-100 Hybrid Detectors*Excellent Dynamic Range*Ultra-High Time Resolution*IRF Width Down to 40 ps FWHM with HPM-100-06/07*Low Cost Detector Option: PMC-150 PMT Modules
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Multiphoton FLIM System
DCS-120 MP
*Multiphoton FLIM Upgrade for Existing Conventional Microscope*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Scanning by Fast Galvanometer Mirrors*Channel Seperation by Dichroic or Polarising Beamsplitters*Individually Selectable Pinholes and Filters*Excitation by Ti:Sa Laser*Two Non-Descanned Detection Channels*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: Two HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Electronic Pinhole Alignment*Z-Stack FLIM Acquisition with Zeiss Axio Observer Z1*Optional: Spatial Mosaic FLIM via Motorized Sample Stage*Laser Control via SPCM Software
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Confocal FLIM for Macroscopic Objects
DCS-120 Macro
*FLIM of Macroscopic Objects*Scan Field Up to 15 mm Diameter*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Scanning by Fast Galvanometer Mirrors*Channel Seperation by Dichroic or Polarising Beamsplitters*Individually Selectable Pinholes and Filters*One or Two BDL-SMN or BDS-SM ps Diode Lasers*Tuneable Excitation by Super-Continuum Laser with AOTF*Two Fully Parallel Detection Channels*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: Two HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Wideband Version, Compatible with Tuneable Lasers*Electronic Pinhole Alignment*Optional: Spatial Mosaic FLIM via Motorized Sample Stage
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Picosecond-Diode Lasers for Spectroscopy
Designed and manufactured by Becker & Hickl – bh delivers prominent picosecond diode lasers with wavelengths from the NUV to the NIR. All bh pulsed diode lasers work with simple +12V power supply or pull their power from the USB port of a PC or Notebook Computer. Other features are high repetition rate, short pulse width, unprecedented timing and power stability, and extremely low electrical noise level.
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High Voltage Breakdown Test Sets
HT-Series
Dielectric Voltage Withstand Test or High Potential Test (HIPOT test) is a 100% production line test to verify the quality & electrical safety characteristics of products.
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Laser
BDS-SM Series
*Pulse Width Down to 60 ps*Repetition Rate 20 MHz /50 MHz / 80 MHz / CW (Switchable)*Free Beam or Fiber Output*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*40 mm x 70 mm x 120 mm
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Laser
BDS-MM Series
*Pulse Width Down to 60 ps*Repetition Rate 20 MHz / 50 MHz*Free Beam or Fiber Output*Optical Power Up to 40 mW*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*40 mm x 70 mm x 120 mm
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Instrument Software PMS Modules
PMS
*PMS Standard Software for Data Acquisition*Setting of Measurement Parameters*Control of Measurements for Up to 4 ModulesLoading and Saving of Measurement Data and Parameters*Display of Results as Curves or Bars
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FLIM Upgrade Kits for LSMs
As technology leader in equipment and techniques for single photon counting, Becker & Hickl offers a wide range of high-grade Fluorescence Lifetime Imaging (FLIM) systems for laser scanning microscopes since 1998. Our techniques have found broad application within the scope of Life Sciences, Clinical FLIM, Diffuse Optical Tomography (DOT), Fluorescence Correlation (FCS, FCCS), Förster Resonance Energy Transfer (FRET) and more.
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Analysis Software for FCS and cross-FCS
Burst-Analyzer
*FCS Fit with User-Defined Model Functions*Identification of Single-Molecule Photon Bursts in Parameter-Tag Data*Analysis of Fluorescence Intensity and Lifetime within Photon Bursts*One- and Two-Dimensional Histograms of Burst Parameters*Filtered Histograms of Burst Parameters*Discrimination of Different Fluorescent Species or FRET States*Determination of FRET Efficiencies*Exclusion of Artifacts in Intensity Traces
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FLIM Upgrades for Zeiss LSM
710 / 780 / 880
*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Detection Wavelength Range from 360 nm to 900 nm*Excitation by Ti:Sa Laser, OPO, or by bh ps Diode Lasers*Identical Systems for Multiphoton FLIM and Confocal FLIM*Detectors can be Swapped Between NDD Port and Confocal Port*Systems are Modular: More FLIM Channels can be Added
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Fiber-Based TCSPC System
*Hybrid Fiber System With Implantable Fiber Tip*Tip Removable from Fiber System*Seperate Excitation and Detection Fibers*Extremly Low Background Fluorescence*One or Two bh ps Diode Laser (BDS-SM or BDL-SMN Series)*High-Efficiency HPM-100-40 or -50 Hybrid Detector*Optional: Multi-Wavelength Detector
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Laser
BDL-SMN Series
*Pulse Width Down to 40 ps*Repetition Rate 20 MHz / 50 MHz / 80 MHz / CW (Switchable)*Free Beam Output or Single-Mode Fiber Coupling*Beam Profile Correction Optics*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*Fast On / Off / Multiplexing Capability*Synchronisation Input
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Wide-Field FLIM System
LINCam FLIM
*Easy to Use Like an Ordinal Megapixel CCD Camera*Spatial Resolution ~ 1024 x 1024 Pixels*Temporal Resolution (IRF Width) 50 ps FWHM*Single-Photon Sensitivity*Saturated Count Rate Up to 5 MHz*Excitation by bh BDS-SM Family ps Diode Laser*Works with All Commonly Used MicroscopesI*llumination via Single-Mode Fiber*Lens System for Image Transfer Into Camera
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FLIM Upgrades for Olympus
FV1000
*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excitation by bh ps Diode Lasers*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Detection Wavelength Range from 360 nm to 900 nm*Excitation by Ti:Sa Laser, OPO, or by bh ps Diode Lasers*Systems for Multiphoton FLIM and Confocal FLIM*Systems are Modular: More FLIM Channels can be Added
